TABLE 2.
Quantitative assay | Animal ID | Resulta at indicated time point
|
|||||
---|---|---|---|---|---|---|---|
−42 days | 24 h | 48 h | 72 h | 96 h | 120 h | ||
pagA PCR | A | − | − | + | + | +* | |
B | − | − | − | + | NS | +* | |
C | − | − | + | + | +* | ||
D | − | − | + | − | NS | NS | |
E | − | − | + | + | +* | ||
Bacteremia status | A | − | − | + | − | +* | |
B | − | − | + | ± | NS | +* | |
C | − | − | + | − | +* | ||
D | − | − | + | − | NS | NS | |
E | − | − | + | + | +* |
Qualitative results for pagA PCR are indicated as positive (+) or negative (−). Culture results were graded as positive (+) if there were more than five colonies, negative (−) if there were no colonies, and low positive (±) if there were one to five colonies in primary phase. NS, no sample was available. Asterisks indicate the measurement at euthanasia. Measurements for postexposure time points at 2, 4, 6, and 12 h were negative for all parameters and thus were not included. Due to standard requirements for blood collection during animal studies, samples for animal D from days 6 through 9 were not available for pagA PCR. At euthanasia on day 9, animal D was found to be bacteremic.