FIG. 1.

Recognition of B. bronchiseptica strains RB50 and 1289 using antibodies from convalescent-phase serum by Western blotting and ELISA. Western blots of B. bronchiseptica strain RB50-induced (A) or strain 1289-induced (B) sera were used to determine if antibodies raised against one strain recognized specific antigens of strains RB50, 1289, and RB50Δwbm (A and B). The numbers next to each Western blot indicate molecular weights (in thousands). ELISA was performed on RB50-induced (black), 1289-induced (dark gray), or RB50Δwbm-induced (light gray) serum to quantify titers of antibodies specific for strain RB50, 1289 or RB50Δwbm (C) or purified LPS from strain RB50, 1289 or RB50Δwbm (D). The dashed line represents the lower limit of detection. Asterisks denote P values of <0.05.