FIG. 2.

Western blot analysis using anti-Sab. Strains were grown in LB medium (A and C) or DMEM (B) at 37°C, and lysates or protein extracts were prepared and separated by SDS-PAGE, blotted, and probed with anti-Sab, as described in Materials and Methods. (A) Whole-cell lysates. Lanes: 1, 98NK2sab::kan(pB); 2, 98NK2sab::kan(pBsab); 3, JM109(pB); 4, JM109(pBsab). (B) French press lysates. Lanes: 1, 98NK2; 2, 1:10 dilution of 98NK2; 3, 98NK2sab::kan. (C) Inner and outer membrane fractions. Lanes: 1, 98NK2; 2, 98NK2sab::kan. The arrow indicates the mobility of the 160-kDa Sab species.