FIG. 4.
Surface expression of Sab on whole unfixed cells judged by use of ELISA. Cells from log-phase cultures of 98NK2, 98NK2sab::kan, and 98NK2sab::kan(pBsab) were probed with anti-Sab and secondary goat anti-mouse immunoglobulin G-alkaline phosphatase antibody, as described in Materials and Methods. Surface expression was quantitated by measuring the A405. Data are means ± standard deviations of data from three independent experiments (**, P < 0.01; ***, P < 0.005 by Student's unpaired, two-tailed t test [relative to 98NK2sab::kan]).