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. 2009 Jun 1;77(8):3234–3243. doi: 10.1128/IAI.00031-09

FIG. 6.

FIG. 6.

Protein-binding assay. HEp-2 monolayers were exposed to 0 μg/ml (A) or 20 μg/ml (B) Oregon Green-labeled Sab protein for 2.5 h at 37°C. Unbound protein was removed by washing, and fixed cells were viewed by confocal microscopy.