TABLE 1.
Bacteria and plasmids used in this study
| E. coli strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| 98NK2 | STEC O113:H21 (HUS isolate) | 28 |
| MW10 | STEC O113:H21 (food isolate) | 27 |
| JM109 | E. coli K-12 derivate | 48 |
| JM109(pB) | JM109 carrying pB; Apr | This study |
| JM109(pBsab) | JM109 carrying pBsab; Apr | This study |
| 98NK2sab::kan | 98NK2 sab deletion mutant; Knr | This study |
| 98NK2sab::kan(pB) | 98NK2sab::kan carrying pB; Knr Apr | This study |
| 98NK2sab::kan(pBsab) | 98NK2sab::kan with pBsab; Knr Apr | This study |
| M15(pREP4) | E. coli K-12 derivate | Qiagen |
| M15(pREP4)(pQESab) | M15(pREP4) carrying pQESab; Apr | This study |
| Plasmids | ||
| pB | pBluescript II SK(−) | Fermentas |
| pBsab | sab cloned into pBluescript II SK(−) | This study |
| pQE30 | His6 expression vector | Qiagen |
| pQESab | pQE30 with nucleotides 94 to 2676 of sab | This study |
| pkD4 | kan template for mutagenesis | 9 |
| pkD46 | Red recombinase expression vector | 9 |
a
Knr, kanamycin resistance; Apr, ampicillin resistance.