FIG. 7.

Immunoreactivity with PA and with the 305-319 peptide in sera from rabbits inoculated one time with rAAV vectors expressing PA63. Twelve rabbits received a single inoculation with rAAV vectors expressing PA63. Of these 12 rabbits, 6 also received rAAV expressing LF. Eight weeks after being inoculated, all rabbits were bled and sera were analyzed by ELISA for immunoreactivity with PA, with the peptide sequence comprising aa 305 to 319, or with an irrelevant peptide sequence. For analysis of peptide reactivity, a recombinant protein displaying two copies per molecule of aa 305 to 319 of PA or an irrelevant peptide sequence was immobilized on the ELISA plates as described in Materials and Methods. The lower limit of assay detection for the ELISA is 16; sample data below this limit are plotted at 16. Horizontal lines represent GMTs.