TABLE 1.
Peptide and recombinant proteins used in this study
| Description | Sequence |
|---|---|
| Synthetic peptides | |
| MAP peptidea | GNAEVHASFFDIGGS\ /GNAEVHASFFDIGGS |
| K-K-K | |
| GNAEVHASFFDIGGS/ \GNAEVHASFFDIGGS | |
| PA 305-319 peptide | GNAEVHASFFDIGGS |
| Irrelevant peptideb | DAEFRHDSGYEVHHQK |
| Recombinant proteins | |
| MBP-PA 305-319c | MBP-(GNAEVHASFFDIGGS)2 |
| MBP-PA 299-327c | MBP-(HTSEVHGNAEVHASFFDIGGSVSAGFSNS)2 |
a
The MAP peptide consists of four PA 305-319 peptide arms extending from the α- and ε-amino groups of a branching lysine core indicated by “K-K-K.”
b
The irrelevant peptide sequence is from the Aβ peptide comprising aa 1 to 16, derived from the amyloid precursor protein.
c
The recombinant proteins consist of maltose binding protein (MBP) expressed as fusions with two tandem copies of the peptide sequences shown.