FIG. 2.

Survival defects of various strains in the anaerobic dormancy model or in an anaerobic GasPak chamber sampled for CFU counting at the days indicated. (A) In the anaerobic dormancy model, day 4 is entry into hypoxia and day 6 is 1 day after the onset of anaerobiosis (data not shown.) Statistical significance of differences: *, P < 0.01; **, P < 0.001; ***, P < 0.0001. P values were determined for differences between H37Rv and the mutant strains. Standard deviation is indicated. H37Rv is white, the ΔdosT mutant strain is horizontally striped, the ΔdosS mutant strain is black, the ΔdosS/T mutant strain with constitutively plasmid-expressed dosT is diagonally striped, the ΔdosS/T mutant strain is cross-hatched, the complemented ΔdosS mutant strain is checked, and the complemented ΔdosT mutant strain is gridded. (B) Oxygen utilization in the GasPak chamber by H37Rv, shown by surveying methylene blue decolorization via OD₆₀₀ determination.