TABLE 2.

Primers

Name	Sequence	Unique restriction site
DSKR	5′-CGGATAAATTTTTCCACATAGAGG
UFKFP	5′-CGCAAGATATCGGATTAGGAA
MUT2	5′-GCAGGAGGCCTAGCCAACATTACG	StuI
MUT4	5′-AATGTTGGCTAGGCCTCCTGC	StuI
MUT3	5′-GCAGGAGGCCTAGACAACATTACG	StuI
MUT5	5′-AATGTTGTCTAGGCCTCCTGC	StuI
DFKRP	5′-CGCGGATCCTCATAATATCACGGACCGCAT
stkP-PF	5′-AGGATGCCATATGAGATCTCCTGCAACCATTGCCATT	NdeI
stkP-R2	5′-TTGATTATGAATTCGCTTTTAAGGAGTAGCTGAAGTTG	EcoRI
STKP-RT	5′-GTAGGACAGAATTCAAGACAAGTCTACATAA	EcoRI
STKP-F	5′-AGGATGCCATATGATCCAAATCGGCAA	NdeI
PCL	5′-CCAGCTGAATCCAGGCAGTGAGATTCGTG	EcoRI
PCR	5′-CCATACGTCGACCGGCATTTAATTCTTTTG	SalI