TABLE 5.
Impact of gain-of-function mutation in the CSP receptor ComD on the transformability of stkP mutant strainsa
| Strain | Relevant genotype | Transformed colonies (% Novr/total CFU)
|
|
|---|---|---|---|
| pH 6.5 | pH 8 | ||
| Cp1015 | WT | <0.01 | 0.31 |
| Cp6600 | comD(D299N) | 0.30 | 0.52 |
| Cp6607 | comD(D299N) ΔstkP::cat | 0.25 | 0.62 |
| Cp7000 | ΔstkP::cat | <0.01 | <0.01 |
a
Donor DNA (1 μg/ml) was from strain R119 carrying a nov1 mutation, conferring resistance to 2 μg/ml of novobiocin. Results are representative of three independent experiments. WT, wild type.