Skip to main content
. 2009 May 22;191(15):4888–4895. doi: 10.1128/JB.00285-09

FIG. 3.

FIG. 3.

Mapping of slx by linkage to marinerT7 inserts. (A) Mapping strategy. Top, preparation of a marinerT7 (Ωspc) library in the slx-1 background by transformation. Bottom, cotransfer of a ΔclpX::erm amplicon, slx-1, and Ωspc from the library. (B) Linkage of Ωspc inserts to slx. Frequency of cotransformation of Emr and Spcr from genomic DNA of five Emr Spcr clones that had been obtained by transformation of CP1250 with clpX::erm and the Ωspc slx-1 library donor DNA. Inserts in clones 10,13, 15, 29, and 35 were located at bp 1594927, 1594560, 1605766, 1619303, and 1623486, respectively. (C) Mapping the slx mutation by assay of the slx content of PCR products amplified from CP1361 genomic DNA. Fragments 1, 2, 3, 4, 5, 6, and 7 were amplified with primer pairs DAM850/851, DAM852/853, DAM854/855, DAM856/857, DAM858/859, DAM860/861, and DAM854/853, respectively. Bars indicate the genetic extent of each fragment and the yield of clpX::erm after cotransformation of CP1250 with the indicated fragment and a pure clpX::erm amplicon.