FIG. 5.

Organization of the Slx/spr1630 locus in two natural isolates and a synthetic deletion derivative. (A) Structure of the trpA-cclA region in Hungary19A-6 and other pneumococcal strains lacking spr1630. The segment duplicated in strains carrying spr1630 is indicated by a dotted bracket. (B) Elements adjacent to trpA and cclA in the R6 genome. Diamond, RUPA; triangles, gene remnants in direct repeat. (C) Strategy for deletion of spr1630. PCR products ligated to kan to target insertion by flanking homology are depicted. Small arrows represent primers; small arrows with tails represent primers with 5′ extension and restriction site.