TABLE 2.
Primers used in this study
| Primer name | Gene | Primer sequence (5′-3′)a |
|---|---|---|
| AP22 | clpX | GATAGAATGACAGTATAGAATGGAAGGAAATC |
| AP23 | clpX | ATTTGTAATGAATGCGTGGAGTTAGCTCAG |
| AP24 | clpX | ATGCGTGGAGTTAGCTCAGGAAATCATTCG |
| AP25 | clpX | CTAGGATCGGTTTATCCGTTCCATCGACAG |
| AP26 | clpX | TAGCAATCTCTTGAAGGGCTTCGTCGTCAAATTCC |
| AP27 | clpX | ACGCGCCCCTGTCTTCCGTTCGATTGCTTTATTAG |
| AP30 | spr1422 | CTTGCCTGACTCAGGGTCACGAATG |
| AP33 | lytC | AACGCAAACGAGCGCAGAAGTACAG |
| DAM301 | kan | CGCGCAAGCTGGGGATCCG |
| DAM302 | kan | acgtgggcccTAGGTACTAAAACAATTCATCCAGTAA |
| DAM850 | spr1620 | TGGAAGCTCTGATTCCGCAATTCCG |
| DAM851 | spr1627 | AGGCTTAGGTAGCCAGGCTTTAAGG |
| DAM852 | spr1623 | AGCTGGAGACAGCACTCCGTAGTTG |
| DAM853 | trpA | GGTGACCACGAGAAAGGTTTGGATG |
| DAM854 | spr1630 | GTAACTGACCAGTTCGTGCATCAGG |
| DAM855 | trpE | GAAAGCAACGCTTCCTGCTGGAACC |
| DAM856 | trpD | TCAAGCCAGCTTCATCCAACCCTTC |
| DAM857 | mgtC | TCTGACAACTGCAGCAGGCATTTGG |
| DAM858 | ccpA | AGAACGCACACCTCTTTCACGATCC |
| DAM859 | galEb | TGACAGCGCTTGACAAAGGAAACGG |
| DAM860 | spr1645 | TTGCTAGAATTGCCCGTCCTGTACC |
| DAM861 | spr1650 | ATCAACCGAGTGGGTACTGGATAGG |
| DAM862 | trpA | CACCAAGGAGAGCCGATTCAGGAC |
| DAM863 | spr1629 | CCATGCGCTATAAGCATCAGCTTGG |
| DAM864 | spr1630 | gcaggatccCCTGATGCACGAACTGGTCAGTTAC |
| DAM865 | spr1630 | acagggcccAGGTCGCGTCCATGCATAACCATTC |
| PL46 | erm | GGTCTAGA GGATCCGGGTACCGGGCCCAAAATTTGTTTGATTTGT |
| PL47 | erm | GGGGTACCGAAGCTTGGGATCCGGAATTCAGTCGGCAGCGACTCATAGAATTA |
| PL71 | clpX | gctcTAGACATGATTTCCTTCCATTCTATACTG |
| PL72 | dpr | CGTGTTCTTGTTATCTACCGTTACT |
| PL73 | clpX | cccaagctttcATGTTTGAGGTGCCGAGTCAGGA |
| PL74 | aldR | TTACATCACGAGGAAGACGAGCTAC |
| SA130 | spc | GATCTGTCAATGGTTCAGATAC |
| SA133 | spc | GAAGAGCCATTATGGATTCG |
a
Lowercase segments indicate 5′ extensions; underlined segments indicate restriction sites.
b
Primer binds upstream of galE.