TABLE 3.
Transformation efficiency of ΔclpX::erm in spr1630 mutants in three genetic backgroundsa
| Recipient | DNA donor genotypeb
|
|
|---|---|---|
| novo-r (Novr/ml) | slxΔclpX::erm (Emr/ml) | |
| CP1250 (wild type) | 350,000 | 6,000 |
| CP1973 (slx-1) | 430,000 | 640,000 |
| CP2008 (Δspr1630) | 480,000 | 620,000 |
| R6 | 4,889 | 289 |
| CP2087 (R6 Δspr1630) | 3,511 | 14,000 |
| TIGR4 | 40 | 0.3 |
| CP 2089 (TIGR4 Δspr1630) | 148 | 557 |
a
Exposure (70 min) of cells at OD of 0.06 to 0.1 μg DNA/ml.
b
Novr donor DNA was from CP1500, but from CP2086 for R6 and TIGR4 recipients. Emr donor DNA was from CP1361, but from CP2088 for R6 and TIGR4 recipients.