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. 2009 May 29;191(15):4934–4942. doi: 10.1128/JB.01831-08

FIG. 1.

FIG. 1.

(A) Coomasie blue-stained SDS-PAGE gel of outer membrane preparations of E. coli TOP10 transformed with hra1 in pBR322-pBJ1 (lane 2), the medium-copy-number pBR322 vector (lane 3), hra1 in pGEMT-pTHra (lane 4), and the high-copy-number vector pGEMT (lane 5). Lane 1, prestained marker (Bio-Rad). (B) Anti-Hra1 Western blot of electrophoresed cell lysates of E. coli TOP10 carrying pBR322 (lane 1), hra1 clone pBJ1 (lanes 2 and 3), hra1 with the central 395 bp deleted (pBJ2; lane 4), pBJ2 with an inserted aphA-3 casette (pBJ4; lane 5), and pTHra1 (lane 6).