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Adherence of bacteria to HEp-2 cells in culture after a standard 3-h assay. (A) Uninfected HEp-2 cells; (B) EAEC strain 042, demonstrating classic aggregative adherence; (C) pBR322 vector in E. coli K-12 TOP-10; (D) hra1 clone pBJ1 in TOP-10; (E) hra1 deletion construct, pBJ2, in TOP-10; (F) E. coli K-12 strain MG1655; (G) MG1655 carrying pBJ1; (H) MG1655 carrying pBJ2.
