FIG. 5.

(A) Western blot of whole-cell lysates of 042 (lane 1), hra1 mutant SB1 (lane 2), SB1 complemented with the hra1 clone (pBJ1), pBJ1 in TOP10 (lane 3), and pBJ2, the hra1 deletion construct, in TOP10 (lane 5). (B to D) Autoaggregation in DMEM of 042 (B), its isogenic hra1 mutant (C), and SB1 and the transcomplement SB1(pBJ1) (D) in DMEM. For panel E, biofilm formation by 042, SB1, and SB1(pBJ1) in DMEM was quantified by crystal violet staining and elution. Differences between the wild type and mutant were not significant at any time point (P > 0.05).