TABLE 2.

Oligonucleotide primers used in this study

Primer	Purpose	Sequence	Reference
PUC18KNcoIF	Amplification of apha-3 cassette and attachment of NcoI tails	5′-CATGCCATGGTGACTAAACTAGGAGGAATAAATGGCTAAAATGAGA-3′	This study
PUC18KNcoIR	Amplification of apha-3 cassette and attachment of NcoI tails	5′-CATGCCATGGTCATTATTCCCTCCAGGTACTAAAACAATTCATC-3′	This study
Agg1F	Primer internal to hra1; used to verify mutant	5′-ATTGCGGTTTCAGCGCTTGC-3′	This study
Agg1R	Primer internal to hra1; used to verify mutant	5′-AGATAGCGATAGCTGAGGTC-3′	This study
Hra1upF	Upstream of hra1 but absent from hra1 deletion construct pSB1; used for primary cloning of the gene and to verify mutant	5′-GCAAATCACAACCTTCCTGA-3′	This study
Hra1dnR	Downstream of hra1 and present in hra1 deletion construct pSB1; used for primary cloning of gene and to verify mutant	5′-CTTCGGAGTGGATGTATCTG-3′	This study
AA432F	Amplifies portion of aggregative plasmid gene aatA; used to verify mutant	5′-CTGGCGAAAGACTGTATCAT-3′	8
AA432R	Amplifies portion of aggregative plasmid gene aatA; used to verify mutant	5′-CAATGTATAGAAATCCGCTGTT-3′	8