TABLE 1.
Primer sequences, bacterial strains, and plasmids used and generated in this study
| Primer, strain, or plasmid | Primer sequence | Source or reference |
|---|---|---|
| Primers | ||
| Up ctuF (A) | 5′ CACGCGTCGACACATTCAAATGGTCAAGGAGTTTT 3′ | |
| Up ctuR (B) | 5′ ATCAAATCTCCTTTATAGCCGGT 3′ | |
| Dn ctuF (C) | 5′ CGGCTATAAAGGAGATTTGATATTGATAAAAATGTTTTTAGTTTAGAG 3′ | |
| Dn ctuR (D) | 5′ GGGACTAGTTCATCTAAACGCTAATCATGCTG 3′ | |
| CTUpKKF | 5′ AAAACTGCAGATGGCGAATATAAAAGTTGCAG 3′ | |
| CTUpKKR | 5′ TTAATACTTTCTAACAATTTCTTCA 3′ | |
| pKK kan CTU-F | 5′ TGAAGAAATTGTTAGAAAGTATTAAGTCGACTATTAAAAAAATTCATCAAG 3′ | |
| pKK kan CTU-R PstI | 5′ GGAGTAACTGCAGTATGTCACAT 3′ | |
| Strains or plasmidsa | ||
| F. tularensis Schu S4 | USAMRIID | |
| pDMK | 27 | |
| pDMK::Δctu | This study | |
| S17-1::pDMK::Δctu | This study | |
| F. tularensis Schu S4::Δctu (Δctu) | This study | |
| F. tularensis Schu S4::Δctu+pctu | This study |
a
E. coli S17-1 available in the Center for Immunology and Microbial Disease, Albany Medical College, was used for conjugation experiments.