FIG. 2.

Expression and isoform patterns of Pla and the Pla derivatives in recombinant bacteria. (A) Pla and Pla T259I were expressed in Y. pestis KIMD34, and the Western blot assay shows the reactivity of whole-cell samples with anti-Pla antiserum. (B) Expression and isoforms of the Pla proteins in cell envelope preparations from recombinant E. coli. The protein constructs are indicated above the lanes, and the migration distances of the α, β, and γ isoforms of the mature Pla molecule and of nonprocessed pre-Pla are indicated on the left. The plus sign denotes samples boiled for 10 min before electrophoresis, and the minus sign denotes unboiled samples.