FIG. 5.

Degradation of plasminogen by Pla and PgtE variants expressed in recombinant E. coli XL1. Plg degradation by the bacteria was analyzed by Western blotting with anti-Plg antibody (A) and anti-Plg catalytic domain antibody (B). The migration distances of Plg, plasmin heavy chain (PlnH, apparent size of 70 kDa), plasmin light chain (PlnL, 25 kDa), and angiostatins (Ang) are shown on the left. PlgX indicates a fragment of Plg produced by the bacteria. The Pla and PgtE constructs expressed in E. coli XL1 and uPA-treated Plg are indicated above the lanes, incubation times in panel A are indicated below the lanes. In panel B, the incubation time was 2 h.