TABLE 2.
Primers used in this study
| Primer name | Sequence (5′ → 3′) | Nucleotide positiona |
|---|---|---|
| 5′ lytSR-F-EcoRI | CCCGAATTCTGCAACGGGACAATTGTTAG | 299003-299022 |
| 5′ lytSR-R-BamHI | CCCGGATCCCAACGTGC TTTCCATGTACG | 299530-299549 |
| 3′ lytSR-F-ClaI | TCCACATTTTTTCTTCAAATCGATTAACACGATTTCAGC | 300588-300626 |
| 3′ lytSR-R-PstI | CCAAAAAGTCTGCAGGCTCGATGTCGATTCAAATTGTAATCG | 301102-301143 |
| lytSR-F-BamHI | CCCGGATCCAAACATAATTATAATTCACTGAGGTGCTATCGTGCTATCGC | 299394-299434 |
| lytSR-R-EcoRI | CCCGAATTCCGACCATTGCCTCCTACGTTTG | 302020-302041 |
| lrgA-F | CCCGTTAAATCAAACGTAGGAGG | 302013-302032 |
| lrgA-R | CCCGGTTAATCATGAGCTTGTGC | 302461-302480 |
| cidA-F | CCCCGTATTTAGAAAGGGATGGCGCC | 2706686-2706708 |
| cidA-R | CCCCAAGGCTTGCACGTAATCATTC | 2706274-2706295 |
| LytS-F | TCCAGCAGTTTTTCGCTATGTATAAAAGTC | 299212-299241 |
| LytS-R | TTCCAATGTTTCTTTTACATTTTCTGCCTC | 301272-301301 |
| LytR-F | CCCGGATCCGACAAGAGGAG GAATAAATATG | 301163-301184 |
| LytR-R | CCCGAATTCCGACCATTGCCTCCTACGTTTG | 302020-302041 |
a
Based on the MRSA-252 genome sequence.