FIG. 2.

Visualization of DnaA-EYFP bound to oriC and datA on a low-copy-number plasmid. TetR-mCherry was temporarily induced from a host chromosome that also carries dnaA-eyfp in place of dnaA. In the merged picture at the far right, TetR-mCherry and DnaA-EYFP are shown in magenta and green, respectively. White arrowheads indicate colocalization of DnaA-EYFP and TetR-mCherry foci. Black arrowheads indicate TetR-mCherry foci that are not colocalized with DnaA-EYFP fluorescence. Percentages of the fluorescent spots of DnaA-EYFP that colocalized with the spots of TetR-mCherry out of 100 of the latter surveyed are indicated in parentheses.