FIG. 3.

Characterization of strains used to observe the colocalization of DnaA-EYFP with chromosomal loci. (A) Proportions of cells that harbor each number of LacI-ECFP foci. Strains and positions of LacI-ECFP foci are indicated at the top. One hundred cells were chosen at random for the focus counting. Average focus number per cell is shown. (B) Schematic of the cell cycle of the dnaA-eyfp lacI-ecfp/lacO (ori1) strain in minimal medium at 30°C. LacI-ECFP foci are shown as dots in cells at different stages of the cell cycle. The percentages of cells with each focus number are shown. The percentages in parentheses indicate the proportion of the cells with one to four oriC copies per cell (from left to right) measured by flow cytometry after replication runout. Averages of five experiments are shown with standard deviations.