FIG. 1.

Evidence that K. kingae surface fibers are type IV pili. (A) Fibers on the surface of K. kingae strain 269-492 (left) and the preparation of purified pili (right) after staining with uranyl acetate and examining by transmission electron microscopy. Scale bars represent 100 nM. (B) Coomassie blue-stained SDS-PAGE gel of purified pili (left) and a Western blot of purified pili (right) using the antiserum GP65 directed against PilA. Strain K. kingae 269-492pilF (pilF) is unable to assemble pili and served as a control. MW, molecular size in kilodaltons.