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. 2009 May 22;191(15):4976–4986. doi: 10.1128/JB.00123-09

FIG. 3.

FIG. 3.

Contribution of PilS to K. kingae PilA1 expression and adherence. (A) Western blot for PilA1 in K. kingae strains KK01, 269-492, KK03, 269-492 pilA1, 269-492 pilS, KK01 pilS, and KK03 pilS. NS/NC, nonspreading/noncorroding; SC, spreading/corroding; NP, nonpiliated. (B) Levels of pilA1 transcript measured by quantitative real-time PCR in K. kingae strains KK03, KK01, 269-492 pilS, KK01 pilS, and KK03 pilS relative to that of strain 269-492 (*, P ≤ 0.05 using the unpaired t test compared to results for the parental strain). (C) Adherence to Chang respiratory epithelial cells by K. kingae strains 269-492, 269-492 pilS, KK01 pilS, KK03 pilS, and 269-492 pilA1. (D) Adherence to Hig-82 synovial cells by K. kingae strains 269-492, KK01, KK03, 269-492 pilS, KK01 pilS, KK03 pilS, and 269-492 pilA1.