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. 2009 May 22;191(15):4976–4986. doi: 10.1128/JB.00123-09

FIG. 4.

FIG. 4.

Contribution of σ54 to K. kingae PilA1 expression and adherence. (A) Western blot for PilA1 in K. kingae strains 269-492, 269-492 recJ (recJ), 269-492 pilA1 (pilA1), 269-492 rpoN (rpoN), and 269-492 Δ−12 (-12). wt, wild type; S/C, spreading/corroding; NP, nonpiliated. (B) Adherence to Chang respiratory epithelial cells by K. kingae strains 269-492, 269-492 recJ, 269-492 rpoN, 269-492 Δ−12, and 269-492 pilA1 (pilA1). (C) Levels of pilA1 transcript measured by quantitative real-time PCR in K. kingae strains 269-492 rpoN and 269-492 Δ−12 relative to those of strains 269-492 and 269-492 recJ, respectively (*, P ≤ 0.05 using the unpaired t test compared to results for the parental strain).