Contribution of ACA repetitive elements to K. kingae adherence. (A) Western blot for PilA1 in K. kingae strains KK01, 269-492, KK03, 269-492 pilA1 (pilA1), 269-492 ACA#1−, KK01 ACA#1−, KK03 ACA#1−, 269-492 ACA#2−, KK01 ACA#2−, KK03 ACA#2−, 269-492 ACA#1−/ACA#2−, KK01 ACA#1−/ACA#2−, and KK03 ACA#1−/ACA#2−. (B) Adherence to Chang respiratory epithelial cells by K. kingae strains 269-492 recJ, 269-492 ACA#1−, 269- 492 ACA#2−, 269-492 ACA#1−/ACA#2−, KK01 ACA#1−, KK01 ACA#2−, KK01 ACA#1−/ACA#2−, KK03 ACA#1−, KK03 ACA#2−, KK03 ACA#1−/ACA#2−, and 269-492 pilA1. (C) Adherence to Hig-82 synovial cells by K. kingae strains 269-492 recJ, KK01 recJ, KK03 recJ, KK03 ACA#1−, KK03 ACA#2−, KK03 ACA#1−/ACA#2−, and 269-492 pilA1.