TABLE 1.
Strains used in this study
| Strain | Description | Reference or source |
|---|---|---|
| 269-492 | K. kingae isolate from St. Louis Children's Hospital | 19 |
| KK01 | Nonspreading/noncorroding variant of strain 269-492 | 19 |
| KK03 | Spreading/corroding variant of strain 269-492 | 19 |
| 269-492 pilF | Strain 269-492 with an aphA3 insertion in pilF | 19 |
| 269-492 pilA1 | Strain 269-492 with an aphA3 insertion in pilA1 | 18 |
| 269-492 recJ | Strain 269-492 with an aphA3 insertion in recJ | 18 |
| KK01 recJ | Strain KK01with an aphA3 insertion in recJ | This work |
| KK03 recJ | Strain KK03 with an aphA3 insertion in recJ | This work |
| 269-492 rpoN | Strain 269-492 with an aphA3 insertion in rpoN | This work |
| 269-492 Δ−12 | Strain 269-492 with an aphA3 insertion in recJ and replacement of the σ54 −12 DNA GC binding region with AA | This work |
| 269-492 pilS | Strain 269-492 with an aphA3 insertion in pilS | This work |
| KK01 pilS | Strain KK01 with an aphA3 insertion in pilS | This work |
| KK03 pilS | Strain KK03 with an aphA3 insertion in pilS | This work |
| 269-492 pilR | Strain 269-492 with an aphA3 insertion in pilR | This work |
| KK01 pilR | Strain KK01 with an aphA3 insertion in pilR | This work |
| KK03 pilR | Strain KK03 with an aphA3 insertion in pilR | This work |
| 269-492/ACA#1− | Strain 269-492 with an aphA3 insertion in recJ and replacement of ACA#1 repetitive element with random DNA | This work |
| KK01 ACA#1− | Strain KK01 with an aphA3 insertion in recJ and replacement of ACA#1 repetitive element with random DNA | This work |
| KK03 ACA#1− | Strain KK03 with an aphA3 insertion in recJ and replacement of ACA#1 repetitive element with random DNA | This work |
| 269-492/ACA#2− | Strain 269-492 with an aphA3 insertion in recJ and replacement of ACA#2 repetitive element with random DNA | This work |
| KK01 ACA#2− | Strain KK01 with an aphA3 insertion in recJ and replacement of ACA#2 repetitive element with random DNA | This work |
| KK03 ACA#2− | Strain KK03 with an aphA3 insertion in recJ and replacement of ACA#2 repetitive element with random DNA | This work |
| 269-492/ACA#1−/ACA#2− | Strain 269-492 with an aphA3 insertion in recJ and replacement of ACA#1/ACA#2 repetitive elements with random DNA | This work |
| KK01 ACA#1−/ACA#2− | Strain KK01 with an aphA3 insertion in recJ and replacement of ACA#1/ACA#2 repetitive elements with random DNA | This work |
| KK03 ACA#1−/ACA#2− | Strain KK03 with an aphA3 insertion in recJ and replacement of ACA#1/ACA#2 repetitive elements with random DNA | This work |
| DH5α | E. coli F− φ80dlacZΔM15 Δ(lacZYA-argF)U169deoRrecA1endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 | 29 |
| DH5α MalC2 | DH5α containing pMalC2, which expresses MBP | This work |
| DH5α MAL-PilR | DH5α containing pMalC2:pilR, which expresses MBP-PilR | This work |
| DH5α pilA1:ΔSS | DH5α containing pHAT10/pilA1:ΔSS, which expresses HAT-PilA1ΔSS | This work |