TABLE 2.
Primers used in this study
| Name | Sequencea |
|---|---|
| PilSk/orev | GCATGGATCCGTCGTTGGCTTGGCGCATGGC |
| PilSk/ofwd | GCATGGATCCGCAGCCTGCAACATTTGTTAGCCG |
| pilRRev | ACGTGTCGACCATATTGATTATTCAATATTCAAGCGTTCC |
| pilRFwd | ACGTGAATTCATGACCAAACACAGTTTGAATCGCC |
| pilRk/oFwd | ACGTACGCGTGCGAAAGCGGTTCGGGTAAAGAG |
| pilRk/oRev | ACGTACGCGTCCGAAATATAGACAGGCACAACGGC |
| ΔpilA-12fwd | CAAATGGCATGCACTCTAATACCAAGTAAGGCAAGTAACACAGC |
| ΔpilA-12rev | GCTGTGTTACTTGCCTTACTTGGTATTAGAGTGCATGCCATTTG |
| ΔACA#1Fwd | ATCGTGAGATCTGTGACTGAGGTCTTGGCATGCACTCTGCTACCAAGTAAGGCAAGTAAC |
| ΔACA#1Rev | ATCGTGAGATCTGTGGATCGGCTAGAATGGTATTTTATTAAACTGCCTTTTTTTGTCAGTCGCAC |
| ΔACA#2Fwd | ATCGTGACTAGTGCACTGCGTCGGCGGCACGTTGATAAGCGAATTGCACCCAAATTG |
| ΔACA#1Rev | ATCGTGACTAGTGGTCTAGCGTCGAAAATAGCGCGCTGATTGAAGCCTGCAATCTG |
| SRLFwd | ATGCATGGTACCAGAAAGCGAATTGAACGCGTTGC |
| SRLRev | ATGCATGTCGACGCTTTCACGTCTTCGGGCAGC |
| pilRDSBF | ATCGGGATCCTTTTTGATTTGGGTATAAAAGGGTATGCCGTAGG |
| pilRDSBR | ATCGGGATCCTTTGGACAAAAGGGCAAAAAGCAGCC |
| PilinFΔSS | TACGGGATCCAGATTACACCAAACGTTCACGCGTATCTGA |
| PilinR | TACGGAATTCTTAGCCACCATTGGTAGCGGTAGTAGTGCC |
| pilA1pdr-6fam | FAM-CTCAGTCAAAGCACTTTTCACG |
| pilA1pdr | CTCAGTCAAAGCACTTTTCACG |
| pilA1pdf | GAGCAGGGAACAGATTGCAGG |
| Pilin Region Fwd | ACGTGAATTCAAGCGCGTATGCCGTGCGAC |
| Pilin Region S3 | GCCGATTATGATGCCGATGGC |
| pilA1 RT-left | CAAACGTTCACGCGTATCTG |
| pilA1 RT-right | GGCCATGCATTGTTAGAAGC |
| ftsZ RT-left | CCAGAGCGAACCAAAGTCTC |
| ftsZ RT-right | AAGCTATACTCGCCCTGCTG |
| pillR RT-left | GTTGATGTCCGCATTGTGTC |
| pilR RT-right | GGCGATAGAACAGGTCTTGG |
| moxR RT-left | TTTATGTTGGCGGACGAAAT |
| moxR RT-right | CGTCCACCGATACTTGACCT |
| raiA-right | GCGGTTACCTTGTCGGTAGA |
| raiA-left | TCTTCCACTGCTTCAACGTG |
a
FAM, 6-carboxyfluorescein.