TABLE 1.

Kinetic parameters of oleate hydratase at different mixing speeds and temperatures^a

Mixing speed (rpm)	Temp (°C)	Apparent Vmax (mU/mg)	Apparent Km (mM)
1,000	30	170 ± 90	0.21 ± 0.99
1,400	30	140 ± 15	0.30 ± 0.26
1,400	22	160 ± 90	0.121 ± 1.0
2,200	22	390 ± 70	0.56 ± 0.54
2,200b	22	340 ± 40	0.078 ± 2.2

^aV_max and K_m were determined from Michaelis-Menten fits of initial rate data points that were determined at three different apparent substrate concentrations (2 mM, 4 mM, and 8 mM), each in at least two independent experiments.

^bData points obtained in a single experiment in the presence of 2.5% isopropyl alcohol.