FIG. 2.

FlgM expression in various H. pylori flagellar basal body mutants. FlgM expression in H. pylori wild-type strains (HPN6 and HP88) and defined isogenic mutants in flagellar regulatory and basal body genes (whole-cell lysates; see Materials and Methods for descriptions of the mutants) and flgM mutant (negative control). M, molecular mass marker. Approximately 10 μg of total cell lysate protein for the upper panel or 30 μg for the lower panel was loaded in each lane. Immunodetection was performed using anti-H. pylori FlgM antiserum (1:300).