TABLE 1.
Strains used in this study
| Strain | Genotypea | Reference |
|---|---|---|
| H. pyloristrains | ||
| 26695 | Wild-type strain | 45 |
| 88-3887 | Motile variant of 26695 | 21 |
| N6 | Wild-type stain | 13 |
| N6flhA | N6 flhA::aphA3′-III | 39 |
| N6flgM | N6 flgM::aphA3′-III | 24 |
| N6fliA | N6 fliA::aphA3′-III | 24 |
| N6fliI | N6 fliI::aphA3′-III | This study |
| N6rpoN | N6 rpoN::aphA3′-III | 33 |
| 88-3887flgM | 88-3887 flgM::aphA3′-III | 24 |
| 88-3887flhA | 88-3887 flhA::aphA3′-III | 33 |
| 88-3887fliP | 88-3887 fliP::aphA3′-III | 21 |
| 88-3887fliI | 88-3887 fliI::aphA3′-III | This study |
| 88-3887fliF | 88-3887 fliF::aphA3′-III | This study |
| N6flhF | N6 flhF::aphA3′-III | 33 |
| N6(pCJ604) | N6 + plasmid pCJ604 | This study |
| N6flgM(pCJ604) | N6 flgM::aphA3′-III + plasmid pCJ604 (FlgM-GFP) | This study |
| N6fliA(pCJ604) | N6 fliA::aphA3′-III + plasmid pCJ604 (FlgM-GFP) | This study |
| N6flhA(pCJ604) | N6 flhA::aphA3′-III + plasmid pCJ604 (FlgM-GFP) | This study |
| N6fliI(pCJ604) | N6 fliI::aphA3′-III + plasmid pCJ604 (FlgM-GFP) | This study |
| N6flgM(pCJ607) | N6 flgM::aphA3′-III + plasmid pCJ607 (FlgM-V5) | This study |
| E. coli strains | ||
| DH5α | F−endA1 recA1 hsdR17 Δ(lacZYA-argF)U169 thi1 supE44 gyrA96 relA1 | 17 |
| ER2566 | F− λ−fhuA2[Ilon], ompT lacZ::T7 gene I gal sulA11Δ(mcrC-mrr)11::IS10 R (mcr-73::miniTn10)2 R(zgb-210)::Tn10)1 (Tets) endA1 [dcm] | New England Biolabs |
| MC1061 | F−araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Strr) hsdR2 (rK+ mK+) mcrA mcrB1 | 5 |
| TG1 | Expression of FlhAC | 38 |
| BTH101 | Cya-BACTH expression strain; F−cya-99 araD139 galE15 galK16 rpsL1 (Strr) hsdR2 mcrA1 mcrB1 | 25 |
a
Kmr (aphA3′-III), kanamycin resistance cassette (28).