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. 2009 May 22;191(15):4916–4923. doi: 10.1128/JB.00407-09

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FIG. 5. — SDS-polyacrylamide gel electrophoresis of histidine-tagged BpsA used for the in vitro assays (A) and kinetic analysis of the enzyme (B). (A) SDS-polyacrylamide gel electrophoresis of histidine-tagged BpsA purified from E. coli BL21(DE3) harboring pColdI-bpsA. Lane 1, size markers including ovalbumin (45 kDa) and carbonic anhydrase (30 kDa); lane 2, the soluble fraction of the crude extract; lane 3, the insoluble fraction; and lane 4, histidine-tagged BpsA purified with a Ni-nitrilotriacetic acid spin column. (B) The 15-methylhexadecanoyl-NAC concentration profile for the synthesis of 4-hydroxy-6-(14′-methylpentadecyl)-2-pyrone (2e) from 15-methylhexadecanoyl-NAC and malonyl-CoA are shown. The data were obtained from three independent experiments.