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. 2009 Jun 3;83(16):8198–8207. doi: 10.1128/JVI.02549-08

FIG. 2.

FIG. 2.

Different susceptibilities of NKG2D ligands to MCMV. (A) NIH 3T3 and SVEC4-10 cells were infected for 12 h with one PFU of WT-gfp MCMV/cell or left uninfected. Cells were stained with PE-labeled NKG2D tetramer (filled histograms). AV-PE was used as a control (open histograms). (B) Expression of NKG2D ligands on SVEC4-10 cells was tested by specific MAbs: rat anti-MULT-1, mouse anti-RAE-1δ, rat anti-RAE-1ɛ, rat anti-RAE-1αβγ, and rat anti-H60, followed by biotinylated-goat anti-rat IgG or biotinylated-goat anti-mouse IgG and PE-labeled streptavidin (filled histograms). Isotype-matched rat IgG2a and mouse IgG1 MAbs were used as a negative control (open histograms). (C) SVEC4-10 and (D) C3H/J derived MEFs were infected for 12 h with one PFU of WT-gfp MCMV/cell or left uninfected. Cells were stained with PE-labeled NKG2D tetramer or with MAbs to RAE-1δ, RAE-1ɛ, and MULT-1 (filled histograms). Isotype-matched irrelevant MAbs and AV-PE were used as controls for staining with specific antibodies and tetramer, respectively (open histograms).