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. 2009 Jun 3;83(16):8198–8207. doi: 10.1128/JVI.02549-08

FIG. 4.

FIG. 4.

The PLWY motif of RAE-1 protein modulates sensitivity to MCMV. (A) Comparison of the amino acid sequences of RAE-1 isoforms using the Vector NT AlignX program (NCBI Pub-Med database search). The PLWY motif (amino acids 49 to 52) is marked. (B) RAE-1γ-, RAE-1δ-, and RAE-1δ-PLWY-transfected NIH 3T3 cells were infected for 12 h with the indicated viruses and analyzed for the expression of RAE-1 isoforms using the anti-FLAG M2 MAb. (C and D) RAE-1δ- and RAE-1δ-PLWY-transfected NIH 3T3 cells were infected for 12 h with four PFU of indicated viruses/cell and analyzed either by immunoblotting (IB) using the anti-RAE-1 MAbs after the immunoprecipitation (IP) with anti-FLAG-M2 Sepharose (C) or by confocal microscopy (D).