FIG. 11.

Effects of seven sequence insertions (duplications) discovered in MUTΔ1-284-derived pseudorevertants on RNA replication efficiency. Naïve BHK-21 cells were mock transfected (Mock) or transfected with the synthetic RNAs transcribed from WT cDNA or one of the reconstructed MUTΔ1-284 derivative cDNAs, as indicated. (A) Representative focus morphology. (B) Production of JEV RNA. (C) Accumulation of JEV proteins. (D) Production of infectious virions. Experiments were performed as described in the legend to Fig. 9.