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. 2009 Jun 3;83(16):7909–7930. doi: 10.1128/JVI.02541-08

FIG. 12.

FIG. 12.

Cell type-dependent replication of MUTΔ1-381-derived mutant virus. (A) Viral growth properties in human neuroblastoma SH-SY5Y and mosquito C6/36 cells. Monolayers of SH-SY5Y or C6/36 cells were infected at an MOI of 1 with the WT or one of the five 3′NCR mutant viruses derived from the respective cDNAs, as indicated. At various time points postinfection, aliquots of culture supernatants were collected and used for virus titration on naïve BHK-21 cells. Data were consistent in two independent experiments. (B) Viral protein accumulation in infected C6/36 cells. Monolayers of C6/36 cells were mock infected or infected at an MOI of 1 with WT, MUTΔ1-284-derived, or MUTΔ1-381-derived virus, or at an MOI of 10 with MUTΔ1-381-derived virus in parallel. At 48 and 144 h postinfection (hpi), the levels of JEV-specific proteins or JEV NS1 protein were analyzed by immunoblotting with the anti-JEV antiserum (anti-JEV) or a rabbit antiserum specific for JEV NS1 (anti-NS1), respectively. The positions of the viral proteins (E, NS1, and NS3) and a cleavage-related intermediate (open arrowhead) are indicated on the left. Molecular size markers (in kilodaltons) are shown on the right.