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. 2009 Jun 3;83(16):7909–7930. doi: 10.1128/JVI.02541-08

FIG. 4.

FIG. 4.

Identification of a novel 83-nt sequence duplication in the 3′-terminal region of the genomic RNAs extracted from MUTΔ1-450-derived pseudorevertants (MUTΔ1-450/Rev). (A) Relative location of a newly acquired 83-nt sequence (83-nt Ins; gray box) duplicated from the 3′-terminal region of the viral ORF in the 3′-terminal region of the MUTΔ1-450/Rev RNAs. (B) Nucleotide sequences of the 3′-terminal region of the original MUTΔ1-450 RNA (left) and its pseudorevertant MUTΔ1-450/Rev RNA (right). The uppercase sequences indicate the 3′-terminal sequences of the viral ORF; the lowercase sequences represent the entire 3′NCR of the corresponding RNAs. The 5′-end nucleotide of the 3′NCRs is numbered nt 451, and bases extending downstream are assigned consecutively according to the WT sequence. Also indicated are the translation stop codons UAG (open arrowheads), the 25-nt CS1 motif (curved lines), and the 8-nt core sequence of the 3′CYC motif (solid arrowheads). Highlighted only in MUTΔ1-450/Rev RNA is the newly discovered 83-nt sequence (double straight lines) originating from the corresponding 3′-terminal region (single straight lines) of the viral ORF.