TABLE 1.
Env processing, virion incorporation, and fusogenic potentials
| Env | Ratioa
|
% Entry relative to that of WT Envb | |
|---|---|---|---|
| gp160/gp120 | gp120/p24 | ||
| SF162 | |||
| WT | 0.52 | 1.00 | 100.00 |
| V1FLAG-a | 1.02 | 1.38 | 71.64 |
| V1FLAG-b | 0.62 | 2.33 | 74.08 |
| V1FLAG-c | 0.83 | 1.10 | 31.19 |
| V1HA-a | 0.69 | 0.53 | 27.72 |
| V1HA-b | 0.46 | 0.68 | 37.44 |
| V1HA-c | 0.63 | 1.73 | 10.34 |
| V2FLAG-a | 1.10 | 1.76 | 17.28 |
| V2FLAG-b | 1.91 | 1.55 | 3.97 |
| V2FLAG-c | 3.22 | 1.58 | 4.02 |
| V2HA-a | 2.41 | 0 | (−) |
| V2HA-b | 2.08 | 0.64 | 1.67 |
| V2HA-c | 3.39 | 2.69 | (−) |
| V4FLAG-a | 5.85 | 0.19 | (−) |
| V4FLAG-b | 1.28 | 3.74 | 24.75 |
| V4FLAG-c | 5.16 | 1.37 | (−) |
| V4HA-a | 11.38 | 0.30 | (−) |
| V4HA-b | 1.10 | 1.46 | 23.51 |
| V4HA-c | 9.96 | 0.15 | (−) |
| SF33 | |||
| WT | 0.73 | 1.00 | 100.00 |
| V1FLAG-a | 1.28 | 0.87 | 10.25 |
| V1FLAG-b | 1.45 | 0.58 | 41.95 |
| V1FLAG-c | 0.75 | 1.05 | 100.93 |
| V1HA-a | 0.96 | 1.00 | 11.80 |
| V1HA-b | 0.55 | 1.07 | 18.47 |
| V1HA-c | 0.86 | 1.25 | 4.42 |
| V2FLAG-a | 1.55 | 0.74 | (−) |
| V2FLAG-b | 1.66 | 0.78 | (−) |
| V2FLAG-c | 0.97 | 0.72 | 8.29 |
| V2HA-a | 14.13 | 0.13 | (−) |
| V2HA-b | 1.27 | 0.61 | (−) |
| V2HA-c | 1.68 | 0.32 | 0.80 |
| V4FLAG-a | >20 | 0.24 | (−) |
| V4FLAG-b | 0.65 | 0.73 | 19.25 |
| V4FLAG-c | >20 | 0.34 | (−) |
| V4HA-a | >20 | 0.31 | (−) |
| V4HA-b | 0.73 | 1.21 | 32.69 |
| V4HA-c | >20 | 0.21 | (−) |
a
gp160/gp120 ratios are for cell-associated Env; gp120/p24 ratios are for virion-associated gp120.
b
(−), <1% of WT entry.