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Gp120 expression of FLAG and HA tags
| Env | Endpoint titer (μg/ml)a of the following MAb:
|
|||
|---|---|---|---|---|
| 2G12 | Anti-FLAG | Anti-HA | Anti-tag/2G12 | |
| SF162 | ||||
| WT | 0.0501 | NA | NA | NA |
| V1FLAG-b | 0.0404 | 0.7134 | — | 17.66 |
| V4FLAG-b | 1.8764 | NA | — | NA |
| V1HA-a | 0.1834 | — | NA | NA |
| V4HA-b | 1.0312 | — | NA | NA |
| SF33 | ||||
| WT | 0.0103 | 40.427 | NA | 3924.95 |
| V1FLAG-b | 0.0284 | 1.8337 | — | 64.57 |
| V4FLAG-b | 0.0134 | 13.91 | — | 1038.06 |
| V1HA-b | 0.0153 | — | 0.0169 | 1.10 |
| V4HA-b | 0.0181 | — | NA | NA |
NA, the highest titer was below the endpoint cutoff; —, ELISA was not done.
