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M33-induced CREB activation is mediated by PKC. (A) HEK293 cells stably transfected with empty vector (mock) or M33FLAG were treated with the PKC inhibitor Ro-32-0432 (0.1 or 0.5 μM for 24 h) and CREB-driven luciferase activity was measured and normalized to Renilla luciferase. (B) Expression of M33FLAG was confirmed by Western blotting with an anti-FLAG polyclonal antibody. The data represent the means ± the SE of three independent experiments performed in duplicate. **, P ≤ 0.005.
