FIG. 8.
M33 activates the Gq/11/PLC-β pathway in salivary epithelial cells. (A) SGC1 salivary epithelial cells were transduced with the retroviruses MIGR1, MIGR1-M33FLAG(WT), or MIGR1-M33FLAG(R131A). PLC-β activity was then measured in each cell line and is expressed as the percent conversion of input myoinositol into inositol phosphates. The data represent the means ± the SE of five independent experiments performed in duplicate. (B) Expression of M33FLAG and M33FLAG(R131A) in the transduced SGC1 cells was confirmed by Western blotting with an anti-FLAG polyclonal antibody (upper panel).