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. 2009 Jun 3;83(16):8090–8098. doi: 10.1128/JVI.02379-08

FIG. 2.

FIG. 2.

Camptothecin inhibits Zta-dependent transcription activation. (A) ZKO-293 cells were transfected with Zta and then treated with 1 μM camptothecin or mock treated. Zta binding to the viral promoters Hp, Rp, and Mp was assayed by ChIP with antibody to Zta (αZta) or with control IgG. ChIP DNA was quantified by real-time PCR and presented as change over IgG. (B) Same as in A except that ChIP assays were performed with antibody to Topo I. (C) 293 cells were transfected with Hp-Luc with either Zta expression vector (+) or control vector (−). Cells were then treated with 0, 0.25, or 1.0 μM camptothecin (CTN), as indicated. Luciferase assays were performed at 48 h posttransfection. (D) Same as in C except for the Mp-Luc reporter. (E and F) Western blot control for Zta expression levels for luciferase assays shown in C and D, respectively.