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. 2009 Jun 3;83(16):8090–8098. doi: 10.1128/JVI.02379-08

FIG. 3.

FIG. 3.

Depletion of Topo I inhibits Zta-mediated lytic replication. (A) ZKO-293 cells were transfected with siRNA targeting Topo I (siTopo I) or control siRNA at 100 or 200 pmol per transfection. Topo I expression levels were assayed by Western blotting. (B) ZKO-293 cells transfected as described above (A) were assayed for EBV genome copy numbers using real-time PCR analysis of EBV OriLyt DNA relative to cellular actin DNA. (C) 293 cells were transfected with Hp-Luc and either Zta expression vector (+) or control vector (−). Cells were then transfected with siRNA targeting Topo I (siTopo I) or control siRNA (siControl) as indicated. Luciferase assays were quantified as the mean values for three independent transfections and errors for standard deviations from the means. RLU, relative light units.