FIG. 1.

Identification of K1 as an interacting partner of MX LCMV NP. (A) Pull-down assay. GST-NP fusion protein and GST were immobilized on glutathione-S Sepharose and incubated with extracts from HeLa cells. Proteins bound to GST-NP and GST were separated by SDS-PAGE, and whole extracts were loaded for the control of input. A prominent band corresponding to 70 kDa was detected among GST-NP pulled-down proteins (arrow) and identified by sequencing as K1. (B) Pull-down assay combined with Western blotting and immunodetection of K1. (C) Immunoprecipitation combined with Western blotting was performed as a proof of interaction between virus NP and K1. The antibodies used for each step are indicated at the bottom.