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. 2009 Jun 3;83(16):8082–8089. doi: 10.1128/JVI.00777-09

FIG. 2.

FIG. 2.

Analysis of HSV-1 capsids by sucrose density gradient centrifugation (a), electron microscopy (b), and SDS-PAGE (c). Analysis was performed with capsids isolated by TX-100 treatment of cell-associated virus (lane 1), TX-100 extraction of extracellular virus (lane 2), and capsids isolated from the nuclei of HSV-1-infected cells (lane 3). Bands marked by asterisks in panel a were the ones used for analysis in panels b and c. Note that tegument remains attached to capsids derived from extracellular, but not cell-associated virus.