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. 2009 Jun 10;83(16):8012–8020. doi: 10.1128/JVI.00038-09

FIG. 5.

FIG. 5.

Subcellular localization of HCV (co)receptors and TJ-associated protein ZO-1 is not affected by occludin silencing. Control or occludin shRNA-transduced PLC/PRF/5 cells were processed for immunofluorescence with antibodies against occludin, ZO-1, claudin-1, CD81, and SR-BI. Localization of occludin (red) and HCV (co)receptors or ZO-1 (green) was analyzed by immunofluorescence confocal analysis (the merged projection of confocal stacks is shown by the large labeled panel at the top, and the x-z section is shown by the thin panel at the bottom). Nuclei were stained with DAPI (blue). z sections were compiled by taking 0.5-μm steps through each x-y section. In order to clearly show the obtained results, due to the fact that occludin knockdown was not homogeneous among the occludin shRNA-transduced polyclonal cell population, cells with undetectable levels of occludin are shown. Images are representative of two independent experiments. The black arrows indicate the planes corresponding to the x-z sections shown. Bar, 50 μm.