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. 2009 Jun 10;83(16):8041–8050. doi: 10.1128/JVI.00382-09

FIG. 2.

FIG. 2.

Effect of SM on BHRF1 transcribed from a heterologous promoter. (A) EBV DNA containing the BHRF1 gene including the 5′UTR and intron was cloned into the mammalian expression vector pCDNA3 and transfected into 293 cells with either empty vector plasmid (C) or SM expression plasmid DNA (SM). RNA was isolated 48 h after transfection and analyzed by RT-PCR using BHRF1 primers. One-kbp DNA markers (M) are shown in lane 1. PCR without reverse transcription is shown at right (-RT Control). (B) RNA from samples in panel A above were analyzed by Northern blotting with a BHRF1 probe. Lanes 1 and 2, RNA from 293 cells transfected with BHRF1 and empty vector plasmids (C); Lanes 3 and 4, RNA from 293 cells transfected with BHRF1 and SM expression plasmids (SM). (C) RNA from samples in panel A were probed with glyceraldehyde-3-phosphate dehydrogenase (GAPDH).